详细说明
- Assay TypeSolid Phase Sandwich ELISA
- Format96-well strip plate
- Assay Length4 hours 40 mins (after plate preparation)
- Sample Type & Volume RequiredCell lysates (100 µL)
- Range312.50 - 20,000 pg/mL
- Sufficient MaterialsKits available for two, five, or fifteen 96-well plates*
- SpecificityPlease see the
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15- (96-well) plate pack sizes
- Economical alternative to Western blot
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
- Streptavidin-HRP
PBS: (Catalog # ), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na 2HPO 4, 1.5 mM KH 2O 4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # ), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H 2O 2) and Color Reagent B (Tetramethylbenzidine) (Catalog # )
Stop Solution: 2 N H 2SO 4 (Catalog # )
Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # ), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # ), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
- StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
Superoxide Dismutases, originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Three mammalian isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SODs are typically soluble secreted or cytosolic proteins, but are also found in the mitochondria and extracellular matrix.
Any of three metals, manganese, iron, or copper, may be used in the active site of SOD and are indicative of cellular localization.MnSOD (SOD2) is found in the mitochondria of both prokaryotes and ukaryotes, whereas the cytosol of eukaryotes and prokaryotic organisms contains Cu/ZnSOD (SOD1) and FeSOD, respectively.
There have been several mutations identified in the Cu/ZnSOD (SOD1) gene in amyotrophic lateral sclerosis (ALS) patients, possibly suggesting a role for free radicals in this disease process. The mechanism of motor neuron degeneration that occurs in ALS, however, is unclear at this time. Several hypotheses have been explored for the role of mutant SOD1 and convincing evidence for the involvement of apoptosis has been presented, as well.
- Entrez Gene IDs:6648 (Human); 20656 (Mouse); 24787 (Rat);
- Long Name:Superoxide Dismutase-2
- Aliases:EC 1.15.1.1; indophenoloxidase B; IPOB; IPO-B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn SOD; Mn superoxide dismutase; MnSOD; MVCD6; SOD, Mitochodrial; SOD2; superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2, mitochondrial
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