• Species Reactivity

  Human

• Specificity

  Detects human TNF RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) 4‑1BB, rhBAFF R, rhCD27, rhCD30, rhCD40, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLymophotactin beta  R, rhNGF R, rhOPG, recombinant mouse (rm) OX40, rhRANK, rhTAJ, rhTNF RI, or rmTNF RII.

• Source

  Monoclonal Mouse IgG2a Clone # 22235

• Immunogen

  E. coli-derived recombinant human TNF RII    
  Extracellular domain

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood monocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: TNF RII/TNFRSF1B

TNF RII is a type I transmembrane glycoprotein primarily expressed on hematopoietic cells and is designated TNFRSF1B. TNF RII is a high-affinity receptor for TNF‑ alpha and TNF-beta. A soluble form of the receptor generated by proteolytic cleavage functions as a TNF antagonist.

• Long Name:

  Tumor Necrosis Factor Receptor II

• Entrez Gene IDs:

  7133 (Human); 21938 (Mouse); 156767 (Rat)

• Alternate Names:

  CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II