详细说明
Species Reactivity
Mouse
Specificity
Detects mouse CD151. Stains mouse CD151 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG 2A Clone # 455807
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse CD151
Met1-Tyr253
Accession # BAA22447Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of CD151 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse CD151 APC-conjugated Monoclonal Antibody |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD151
CD151 is a 29 kDa palmitoylated glycoprotein in the tetraspanin superfamily and is expressed by endothelial cells, epithelial cells, megakaryocytes, and platelets. It associates with other tetraspanin proteins and with some integrins including alpha 3/ beta 1, alpha 6/ beta 1, alpha 6/ beta 4, and alpha 7/ beta 1. CD151 modulates cellular adhesion, cell migration, and platelet activation. Mouse CD151 shares 94% - 96% aa sequence identity with human and rat CD151.
Entrez Gene IDs:
977 (Human); 12476 (Mouse); 64315 (Rat)
Alternate Names:
CD151 antigen (Raph blood group); CD151 antigenplatelet surface glycoprotein gp27; CD151 molecule (Raph blood group); CD151; GP27; Membrane glycoprotein SFA-1; MER2; PETA3; PETA-3; PETA-3SFA-1; platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; tetraspanin-24; TSPAN24; tspan-24; TSPAN24hemidesmosomal tetraspanin CD151