• Species Reactivity

  Human

• Specificity

  Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 548908

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human FOLR1    
  Arg25-Met233    
  Accession # P15328

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die   in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the blood and breast milk (11).

• References:

1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.

2. Fowler, B. (2001) Kidney Int. Suppl. 78:S221.

3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.

4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.

5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.

6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.

7. Ross, J.F. et al. (1994) Cancer 73:2432.

8. Parker, N. et al. (2005) Anal. Biochem. 338:284.

9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.

10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.

11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

• Long Name:

  Folate Receptor 1

• Entrez Gene IDs:

  2348 (Human); 14275 (Mouse); 171049 (Rat)

• Alternate Names:

  Adult folate-binding protein; FBP; folate binding protein; folate receptor 1 (adult); Folate receptor 1; folate receptor alpha; Folate receptor, adult; Folbp1; FOLR; FOLR1; FR-alpha; KB cells FBP; MOv18; Ovarian tumor-associated antigen MOv18