详细说明
- Species ReactivityHuman
- SpecificityDetects human IL-23 p19 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) IL-23 heterodimer is observed, less than 10% cross-reactivity with recombinant mouse (rm) IL-23 heterodimer is observed, and no cross-reactivity with recombinant feline IL-23 p19, recombinant canine (rca) IL-23 p19, and recombinant rat IL-23 p19 is observed.. In Western blots, no cross-reactivity with rcaIL-23 p19 or rmIL-23 p19 is observed.
- SourceMonoclonal Mouse IgG 2B Clone # 727753
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenE. coli-derived recombinant human IL-23 p19
Arg20-Pro189
Accession # Q9NPF7 - FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 488
- Recommended
ConcentrationSample
- Intracellular Staining by Flow Cytometry5 µL/10 6 cellsSee below
Intracellular Staining by Flow Cytometry | Detection of IL‑23 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) treated with LPS or (B) untreated, were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # ) and Mouse Anti-Human IL‑23 p19 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC17161G). Quadrant markers were set based on isotype control (Catalog # ). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for . |
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
- References:
- Oppmann, B. et al. (2000) Immunity 13:715.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Parham, C. et al. (2002) J. Immunol. 168:5699.
- Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
- Long Name:Interleukin 23
- Entrez Gene IDs:51561 (Human); 83430 (Mouse)
- Alternate Names:IL23; IL-23; IL23A; IL-23A; IL-23-A; IL-23p19; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha