• Species Reactivity

  Human

• Specificity

  Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 9E10

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  C-terminal region peptide of human c-Myc    
  Ala408-Ala439    
  Accession # P01106

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max.  It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

• Long Name:

  v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)

• Entrez Gene IDs:

  4609 (Human); 17869 (Mouse); 24577 (Rat)

• Alternate Names:

  avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian)