• Species Reactivity

  Human

• Specificity

  Detects human TRA‑1‑85 antigen in flow cytometry.

• Source

  Monoclonal Mouse IgG    ₁ Clone # TRA-1-85

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  2120Ep human embryonal carcinoma cell line

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TRA-1-85/CD147

The TRA-1-85 antigen, also known as OKa blood group antigen, is a specific epitope within the protein known as Basigin, EMMPRIN and CD147. It is a cell surface determinant expressed on almost all human cell types. This antibody has been used in somatic cell hybrid studies to identify tissues of partial human origin (1, 2).

• References:

1. Williams, B.P. et al. (1988) Immunogenetics. 27:322.

2. Spring, F.A. et al. (1997) Eur. J. Immunol. 27:891.

• Entrez Gene IDs:

  682 (Human)

• Alternate Names:

  OKa Blood Group Antigen; TRA185; TRA-1-85