• Species Reactivity

  Human

• Specificity

  Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J.     et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W.     et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S.     et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).

• Source

  Monoclonal Mouse IgG    ₁ Clone # UCHT1

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human thymocytes (1)    
   

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

• References:

1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.

• Entrez Gene IDs:

  916 (Human); 12503 (Mouse)

• Alternate Names:

  CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE