• Species Reactivity

  Mouse

• Specificity

  Detects mouse Nanog in Western blots. In this format, approximately 50% cross-reactivity with recombinant human Nanog is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse Nanog    
  Trp154-Leu262    
  Accession # Q80Z64

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

• Intracellular Staining by Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Nanog

Nanog is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Its expression is high in undifferentiated embryonic stem cells and is down-regulated during embryonic stem cell differentiation, concomitant with loss of pluripotency (1‑3).

• References:

1. Mitsui, K. et al. (2003) Cell 11:631.

2. Chambers, I. et al. (2003) Cell 113:643.

3. Hart, A.H. et al. (2004) Dev. Dyn. 230:187.

• Long Name:

  Nanog Homeobox

• Entrez Gene IDs:

  79923 (Human); 71950 (Mouse)

• Alternate Names:

  FLJ12581; FLJ40451; hNanog; homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox; Nanog