• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Human, mouse, and rat ~180 kDa NMDA NR2B

• Source

  Polyclonal Rabbit Serum

• Purification

  Antigen Affinity-purified

• Immunogen

  NMDA R, NR2B Subunit

• Formulation

  50 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mg/mL BSA and 50% glycerol.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1:1000 dilution

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  For long-term storage,  -20 °C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2B/NMDAR2B

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg  ⁺⁺ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2B form generates a high conductance NMDAR. Human NR2B (also NR3 and GRIN2B) is a 180 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD) and a 646 aa cytoplasmic region. The loop connecting TM segments 2 and 3, plus the N-terminal ECD constitute the glutamate binding site. Human NR2B is more than 98% aa identical to NR2B in mouse, rat, and dog.

• References:

1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.

2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).

3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.

4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.

5. Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.

• Long Name:

  Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B

• Entrez Gene IDs:

  2904 (Human)

• Alternate Names:

  GRIN2B; NMDA R, NR2B Subunit; NMDAR2B; NR2B