• Species Reactivity

  Human, Mouse, Rat, Bovine

• Specificity

  Human, rat, mouse, and bovine ~85 kDa Synapsin I in Western blots.

• Source

  Polyclonal Rabbit Serum

• Purification

  N/A

• Immunogen

  Synapsin I

• Formulation

  50 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1:1000 dilution

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Synapsin I

Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in presynaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat synapsin I is 704 amino acids (aa) in length and contains five domains, named  
A-E/F. Domains A-D lie between aa 1-655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of Ser603 and 566 in domain D, and Ser9 in domain A, reduces the affinity of synapsin I for actin and secretory vesicles.

• Entrez Gene IDs:

  6853 (Human); 20964 (Mouse); 24949 (Rat)

• Alternate Names:

  SYN1; SYN1a; SYN1b; Synapsin 1; Synapsin I; synapsin IBrain protein 4.1; synapsin-1; SYNI