• Species Reactivity

  Human, Mouse, Rat, Bovine, Chicken, Primate, Zebrafish

• Specificity

  Bovine, human, mouse, and rat ~44-47 kDa gamma 2-subunit of the GABAA Receptor in Western blots.

• Source

  Polyclonal Rabbit Serum

• Purification

  Antigen Affinity-purified

• Immunogen

  GABA-A R gamma 2

• Formulation

  50 μL of unpurified rabbit serum.

• Label

  Unconjugated

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Western Blot

  1:1000 dilution

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R gamma 2

GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha,  beta,  gamma, δ,  epsilon,  theta, and π. Three subunits, alpha, beta, and  gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of an alpha, beta  and gamma subunit. The rat gamma 2 isoform is a 48 kDa, 436 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 30-233). The gamma 2 subunit is part of the most common GABAA receptor combination in the mammalian brain ( alpha 1 beta 2 gamma 2). GABA binds at alpha -beta interfaces, while benzodiazepine binds to alpha -gamma interfaces. There are two splice forms, the longest that contains a consensus phosphorylation site in the second cytoplasmic domain, and a short form that shows an absence of this site through a deletion of aa 376-383. PKC phosphorylates the long form at S381, while both the short and long forms are phosphorylated at S365. Phosphorylation blocks receptor activity. gamma 2 subunits are also palmitoylated at multiple sites on cysteines that lie between aa 415-461, facilitating membrane trafficking.

• References:

1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.

2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.

3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.

4. Krishek, B.J. et al. (1994) Neuron 12:1081.

5. Keller, C.A. et al. (2004) J. Neurosci. 24:5881.

6. Moss, S.J. et al. (1992) J. Biol. Chem. 267:14470.

• Long Name:

  gamma-Aminobutyric Acid Type A Receptor, gamma-2 Polypeptide

• Entrez Gene IDs:

  2566 (Human); 14406 (Mouse); 29709 (Rat); 282240 (Bovine)

• Alternate Names:

  CAE2; ECA2; GABA A R gamma 2; GABA(A) receptor subunit gamma-2; GABAAR gamma 2; GABAARg2; GABRG2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; gamma-aminobutyric acid receptor subunit gamma-2; GEFSP3