• Format

  96-well strip plate

• Assay Length

  < 1 hour

• Sample Type & Volume Required Per Well

  Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Citrate Plasma (25 uL), Urine (10 uL)

• Sensitivity

  0.78 umol/L

• Assay Range

  3.1 - 200 umol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)

• Specificity

  Measures Total Nitric Oxide, Nitrite and Nitrate levels in various samples

• Cross-reactivity

  < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

• Interference

  Interference observed with other substances.

Control Available  

<div class="add-to-cart" data-type="anc" data-catbase="QC134" data-return="total-nitric-oxide-and-nitrate-nitrite-parameter-assay-kit_kge001" data-produrl="parameter-immunoassay-control-set-849-for-nitrate_qc134" data-title="Parameter Immunoassay Control Set 849 for Nitrate" data-catnums="QC134">

QC134, Parameter Immunoassay Control Set 849 for Nitrate -

<div class="add-to-cart" data-type="anc" data-catbase="QC135" data-return="total-nitric-oxide-and-nitrate-nitrite-parameter-assay-kit_kge001" data-produrl="parameter-immunoassay-control-set-904-for-nitrite_qc135" data-title="Parameter Immunoassay Control Set 904 for Nitrite" data-catnums="QC135">

QC135, Parameter Immunoassay Control Set 904 for Nitrite -

Product Summary

The Parameter Total Nitric Oxide kit has two assay options. Endogenous nitrite is measured in the first   option. In the second option, nitrate is converted to nitrite using nitrate reductase and total nitrite is measured. To obtain the nitrate concentration, endogenous nitrite is subtracted from the total nitrite value.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
 

Recovery

The recovery of the nitrate standard spiked to levels throughout the range of the assay in various matrices was evaluated.

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of the nitrate standard were serially diluted with the Reaction Diluent to produce samples with values within the dynamic range of the assay.

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

• Alternate Names

  Nitric Oxide;