• Format

  96-well strip plate

• Assay Length

  3.5 hours

• Sample Type & Volume Required Per Well

  Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (50 uL)

• Sensitivity

  0.31 ng/mL

• Assay Range

  0.3 - 20 ng/mL (Cell Culture Supernates, Serum, Plasma, EDTA Plasma, Heparin Plasma, Urine)

• Specificity

  Measures TXB    ₂ levels in various samples

• Cross-reactivity

  Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.

• Interference

  No significant interference observed with available related molecules.

Control Available  

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QC179, Parameter Immunoassay Control Set 931 for Thromboxane B2 -

Product Summary

The Parameter TXB  ₂ Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure   TXB₂ in cell culture supernates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
 

Recovery

The recovery of TXB₂ spiked to levels throughout the range of the assay in various matrices was evaluated.

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TXB  ₂ were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Thromboxane B2

Thromboxane A2 (TXA2), is an eicosanoid involved in platelet aggregation, vasoconstriction, and reproductive functions. However, as TXA2 is rapidly degraded in vivo, TXA2 production is typically examined by assaying the more stable Thromboxane B2 (TXB2) or 11-dehydro-TXB2 (11-d-TXB2) derivatives. Levels of these metabolites are helpful markers in the study of diseases including liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and others.

• Long Name

  Thromboxane B2

• Alternate Names

  Thromboxane B2; TXB2;