• Assay Length

  3.5 hours

• Sample Type & Volume Required Per Well

  Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Homogenates (50 uL), EDTA Plasma (50 uL), Urine (10 uL)

• Sensitivity

  1.54 nmol/L

• Assay Range

  3.8 - 240 nmol/L (Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine)

• Specificity

  Cyclic AMP in mouse and rat samples

• Cross-reactivity

  < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

• Interference

  No significant interference observed with available related molecules.

Control Available  

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QC53, Parameter Immunoassay Control Set 764 for Mouse/Rat cAMP -

Product Summary

The R&D Systems cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure   cAMP in cell culture supernates, cell lysates, tissue homogenates, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine
 

Recovery

The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Preparation and Storage

• Storage

  Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: cAMP

Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.

• Long Name

  Cyclic AMP

• Alternate Names

  cAMP;