• Format

  96-well strip plate

• Assay Length

  2-3 hours

• Sample Type & Volume Required Per Well

  Cell Culture Supernates (150 uL), Cell Lysates (150 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (150 uL)

• Sensitivity

  0.055 uM

• Assay Range

  0.0 - 17 uM

• Specificity

  TBARS in various samples    
   

• Cross-reactivity

  < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

• Interference

  No significant interference observed with available related molecules.

Control Available  

<div class="add-to-cart" data-type="anc" data-catbase="QC164" data-return="tbars-parameter-assay-kit_kge013" data-produrl="parameter-immunoassay-control-set-752-for-tbars_qc164" data-title="Parameter Immunoassay Control Set 752 for TBARS" data-catnums="QC164">

QC164, Parameter Immunoassay Control Set 752 for TBARS -

Product Summary

The Parameter TBARS assay is a 2-3 hour chemical analysis designed to measure TBARS in cell culture   supernates, cell lysates, serum, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
 

Recovery

The recovery of MDA spiked to levels throughout the range of the assay in various matrices was evaluated.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of MDA, acid treated, and serially diluted with deionized water to produce samples with values within the dynamic range of the assay.

Preparation and Storage

• Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

• Alternate Names

  TBARS; Thiobarbituric Acid Reactive Substances;