• Species Reactivity

  Mouse

• Specificity

  Detects mouse 5'‑Nucleotidase/CD73 in direct ELISAs.

• Source

  Monoclonal Rat IgG2a Clone # 496406

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73    
  Trp29-Lys549    
  Accession # Q61503

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 647

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: 5'-Nucleotidase/CD73

CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).

• References:

1. Resta, R. et al. (1993) Gene 133:171.

2. Resta, R. et al. (1998) Immunol. Rev. 161:95.

3. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.

• Entrez Gene IDs:

  4907 (Human); 23959 (Mouse); 58813 (Rat)

• Alternate Names:

  5' nucleotidase (CD73); 5'-NT; 5-NT; 5'Nucleotidase; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase