详细说明
- Species ReactivityMouse
- SpecificityDetects mouse 5'‑Nucleotidase/CD73 in direct ELISAs.
- SourceMonoclonal Rat IgG2a Clone # 496406
- ImmunogenChinese hamster ovary cell line CHO-derived recombinant mouse 5'‑Nucleotidase/CD73
Trp29-Lys549
Accession # Q61503 - FormulationSupplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 350
Applications
- Recommended
ConcentrationSample
- Flow Cytometry0.25-1 µg/10 6 cellsMouse splenocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: 5'-Nucleotidase/CD73
CD73, an ecto-5’-Nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor (1, 2). The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury (3).
- References:
- Resta, R. et al. (1993) Gene 133:171.
- Resta, R. et al. (1998) Immunol. Rev. 161:95.
- Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468.
- Entrez Gene IDs:4907 (Human); 23959 (Mouse); 58813 (Rat)
- Alternate Names:5' nucleotidase (CD73); 5'-NT; 5-NT; 5'Nucleotidase; 5'-Nucleotidase; 5'-nucleotidase, ecto (CD73); CD73 antigen; CD73; E5NT; EC 3.1.3.5; ecto-5'-nucleotidase; eN; eNT; NT; NT55'-nucleotidase; NT5E; NTE; Purine 5-Prime-Nucleotidase
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