• Species Reactivity

  Human

• Specificity

  Detects human NAALADase‑like 2/NAALADL2 in direct ELISAs.

• Source

  Monoclonal Mouse IgG1 Clone # 817225

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human NAALADase‑like 2/NAALADL2    
  Ser152-Asn795    
  Accession # Q58DX5

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 405

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  LNCaP human prostate cancer cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: NAALADase-like 2/NAALADL2

NAALADL2 (N-acetylated alpha -linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444‑596) and a TfR-like dimerization region (aa 688‑777). There are multiple splice forms. One shows a 4 aa substitution for aa 292‑795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314‑795, and a third contains an alternate start site at Met283, accompanied by a deletion of aa 363‑411 and 633‑795. Over aa 152‑795, human NAALADL2 shares 87% and 82% aa sequence identity with mouse and canine NAALADL2, respectively.

• Long Name:

  N-Acetylated Alpha-Linked Acidic Dipeptidase-like 2

• Entrez Gene IDs:

  254827 (Human); 635702 (Mouse); 499583 (Rat)

• Alternate Names:

  inactive N-acetylated-alpha-linked acidic dipeptidase-like protein 2; NAALADase L2; NAALADase like 2; NAALADase-like 2; NAALADL2; N-acetylated alpha-linked acidic dipeptidase 2; N-acetylated alpha-linked acidic dipeptidase-like 2