详细说明
- Species ReactivityHuman
- SpecificityDetects human IL-28 R alpha /IFN-lambda R1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-28 alpha is observed.
- SourceMonoclonal Mouse IgG1 Clone # 601106
- ImmunogenMouse myeloma cell line NS0-derived recombinant human IL-28 R alpha /IFN-lambda R1
Arg21-Ala228
Accession # Q8IU57 - FormulationSupplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 405
Applications
- Recommended
ConcentrationSample
- Flow Cytometry0.25-1 µg/10 6 cellsDaudi human Burkitt's lymphoma cell line and human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-28 R alpha/IFN-lambda R1
IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a widely-expressed type I transmembrane glycoprotein that is cytokine receptor family 2 member 12 (CRF2-12). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R. Its ligands, IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and IL-29 (IFN‑ lambda 1), are mainly produced by antigen presenting cells in response to viruses. The 520 amino acid (aa) mouse IL-28 R alpha contains a 208 aa extracellular domain (ECD) with a fibronectin type III motif. The human IL-28 R alpha ECD shares ~67% aa identity with rat and human IL‑28 R alpha. Isoforms of 491, 437, 322, 283, 244, 211 and 184 aa have alternate N- or C-termini, or lack transmembrane or internal sequences.
- Long Name:Interleukin 28 Receptor alpha
- Entrez Gene IDs:163702 (Human); 242700 (Mouse); 362628 (Rat)
- Alternate Names:class II cytokine receptor CRF2/12; CRF2/12; CRF2-12; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; IFN-lambda R1; IFN-lambda receptor 1; IFN-lambda-R1; IFNLR; IFNLR1; IL-28 R alpha; IL-28 receptor subunit alpha; IL28R alpha; IL-28R1; IL28RA; IL-28Ra; IL-28R-alpha; Interferon lambda receptor 1; interferon lambda, receptor 1; interleukin 28 receptor A; interleukin 28 receptor, alpha (interferon, lambda receptor); interleukin 28 receptor, alpha; interleukin or cytokine receptor 2; interleukin-28 receptor subunit alpha; LICR2; Likely interleukin or cytokine receptor 2
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