详细说明
- Species ReactivityMouse
- SpecificityDetects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or
rhGM-CSF R beta is observed. - SourceMonoclonal Rat IgG2a Clone # 698423
- ImmunogenMouse myeloma cell line NS0-derived recombinant mouse GM-CSF R alpha
Leu30-Pro327
Accession # Q00941 - FormulationSupplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 750
- Recommended
ConcentrationSample
- Flow Cytometry0.25-1 µg/10 6 cellsJ774A.1 mouse reticulum cell sarcoma macrophage cell line
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageStore the unopened product at 2 - 8 °C. Do not use past expiration date.
Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha, respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).
- References:
- Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
- Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
- Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
- Cao, Y. (2007) J. Clin. Invest. 117:2362.
- Park, L.S. et al. (1992) Proc. Natl. Acad. Sci. 89:4295.
- Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
- Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
- Chiba, S. et al. (1990) Cell Regul. 1:327.
- Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
- Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
- Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.
- Long Name:Granulocyte Macrophage Growth Factor Receptor alpha
- Entrez Gene IDs:1438 (Human); 12982 (Mouse)
- Alternate Names:CD116 antigen; CD116; CDw116; colony stimulating factor 2 receptor alpha subunit; colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage); CSF2R; CSF2RA; CSF2RAX; CSF2RAY; CSF2RX; CSF2RY; GM-CSF R alpha; GM-CSF receptor alpha subunit; GMCSFR alpha; GMCSFR; GM-CSFRa; GM-CSF-R-alpha; GMCSFR-alpha; GMR; GMR-alpha; granulocyte-macrophage colony-stimulating factor receptor alpha chain; granulocyte-macrophage colony-stimulating factor receptor subunit alpha; MGC3848; MGC4838