• Species Reactivity

  Human

• Specificity

  Detects human IL-1RAcP/IL-1R3 in direct ELISAs.

• Source

  Monoclonal Mouse IgG1 Clone # 89412R

• Immunogen

  S. frugiperda insect ovarian cell line     Sf21-derived recombinant human IL-1RAcP/IL-1 R3    
  Ser21-Glu359    
  Accession # Q9NPH3

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Human peripheral blood monocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 RAcP/IL-1 R3

IL-1 Receptor Accessory Protein (also IL-1 R3) is a ubiquitous 70-90 kDa member of the interleukin-1 receptor family of proteins (1-5). It serves as a non-ligand-binding accessory component of the receptors for IL-1 alpha, IL-1 beta, and IL-33 (6, 7). Together with IRAK4 and MyD88, it generates a functional signaling complex with IL-1 RI; by itself, it generates a non-signaling, but high-affinity binding complex with IL-1 RII (8). In addition, it interacts with ST2 on mast cells and Th2 T cells to create a functional IL-33 receptor complex (7). Mature human IL-1 RAcP is a type I transmembrane glycoprotein that is 550 amino acids in length. It contains a 347 amino acid (aa) extracellular region (aa 21-367), a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (9). The extracellular region shows three C2-type Ig-like domains, the most membrane proximal of which is suggested to be responsible for dimerization with IL-1 RI (10). There are three alternative splice forms reported for IL-1 RAcP. One is transmembrane and shows a 239 aa substitution for the C-terminal 122 amino acids (11). The other two are soluble; one shows a six aa substitution for aa 351-570, while a second shows a 45 aa substitution for aa 302-579 (12, 13). The soluble receptor isoforms appear to be inhibitory to IL-1 signaling. When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (14). The human and mouse IL-1 RAcP precursors are 89% aa identical; within the extracellular region, they share 86% aa identity.

• References:

   

1. Subramaniam, S. et al. (2004) Dev. Comp. Immunol. 28:415.

2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.

3. Dunne, A. and L.A.J. O'Neill (2003) Sci STKE. Feb 25;2003(171):re3.

4. Huang, J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12829.

5. Greenfeder, S. A. et al. (1995) J. Biol. Chem. 270:13757.

6. Brikos, C. et al. (2007) Mol. Cell. Proteomics 6:1551.

7. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

8. Lang, D. et al. (1998) J. Immunol. 161:6871.

9. SwissProt. Accession # Q9NPH3.

10. Yoon, D-Y. and C.A. Dinarello (1998) J. Immunol. 160:3170.

11. Lu, H-L. et al. (2008) Mol. Immunol. 45:1374.

12. Jensen, L.E. et al. (2000) J. Immunol. 164:5277.

13. Jensen, L.E. and A.S. Whitehead (2003) Cell. Signal. 15:793.

14. Smith, D.E. et al. (2003) Immunity 18:87.

• Long Name:

  Interleukin 1 Receptor Accessory Protein

• Entrez Gene IDs:

  3556 (Human); 16180 (Mouse)

• Alternate Names:

  C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3; FLJ37788; IL-1 R3; IL-1 RAcP; IL-1 receptor accessory protein; IL-1R3; IL-1R-3; IL1RAcP; IL-1RAcP; IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein beta; interleukin-1 receptor accessory protein