详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA) 3]. The binding of Fe(DHBA) 3 results in the quenching of Trp fluorescence in Lipocalin-2. rhLipocalin-2 can bind >1.5 µM of Fe(DHBA) 3 under the described conditions. See Activity Assay Protocol on www.RnDSystems.com
- SourceMouse myeloma cell line, NS0-derived Gln21-Gly198, with a C-terminal 10-His tag
- Accession #
- N-terminal Sequence
AnalysisNo results obtained: Gln21 predicted - Predicted Molecular Mass22 kDa
- SDS-PAGE25-27 kDa doublet, reducing conditions
1757-LC | | |
Formulation Supplied as a 0.2 μm filtered solution in MES and NaCl. | ||
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- Assay Buffer: 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (TCN)
- Recombinant Human Lipocalin‑2/NGAL (rhLipocalin-2) (Catalog # 1757-LC)
- Iron III (Fe3+) (Sigma, Catalog # 16596)
- 2,3,Dihydroxybenzoic Acid (DHBA) (Sigma, Catalog # 126209)
- Ligand Buffer: 0.1 M Tris, pH 8.0
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Prepare a curve of Fe3+ in deionized water with the following serial dilutions: 640, 320, 160, 80, 40, 20, 10, 5, and 2.5 µM.
- Prepare 1 mM DHBA in Ligand Buffer from powder stock.
- Combine equal volumes of the Fe3+ curve with 1 mM DHBA. Include a control containing 1 mM DHBA and Ligand Buffer.
- Incubate at room temperature for 10 minutes. A curve of the metal ligand complex of Fe(DHBA)3 is formed.
- After incubation, perform 5 fold dilutions to the curve using Assay Buffer.
- Dilute rhLipocalin-2 (MW: 21905 Da) to 4 µM in Assay Buffer.
- In the plate, load 50 µL of the diluted Fe(DHBA)3 complex curve and 50 µL of 4 µM rhLipocalin-2.
- Incubate at room temperature for 30 minutes.
- Read at excitation and emission wavelengths of 280 nM and 340 nM, respectively in endpoint mode.
- Plot a 4-parameter curve of Fe(DHBA)3 concentration (x-axis) versus RFUs (y-axis), and calculate a BC50 from the curve.
- Fe(DHBA)3 Curve: 0.001, 0.125, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16, and 32 µM.
- rhLipocalin-2: 2 µM
Members of Lipocalin family share a highly conserved fold with an eight-stranded antiparallel beta barrel, and act as a transporters, carrying small molecules to specific cells (1). Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), was originally identified as a component of neutrophil granules (2). It is a 25 kDa protein existing in monomeric and homo- and heterodimeric forms, the latter as a dimer with human neutrophil gelatinases (MMP-9) (2). Its expression has been observed in most tissues normally exposed to microorganism, and its synthesis is induced in epithelial cells during inflammation (3). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (Kd = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less Kd values (7.9 nM) (6). Secretion of Lipocalin‑2 in immune cells increases by stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).
- References:
- Flower, D.R. et al. (1994) FEBS Lett. 354:7.
- Kjeldsen, L. et al. (1993) J. Biol. Chem. 268:10426.
- Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
- Devireddy, L.R. et al. (2001) Science 293: 829.
- Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
- Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
- Flo, T.H. et al. (2004) Nature 432:917.
- Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
- Long Name:Neutrophil Gelatinase-associated Lipocalin
- Entrez Gene IDs:3934 (Human); 16819 (Mouse); 170496 (Rat)
- Alternate Names:24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin