• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED    ₅₀ for this effect is 0.8-4 µg/mL in the presence of 10 ng/mL of rhNGF.

• Source

  Mouse myeloma cell line, NS0-derived

  Human Trk A (Ala33-Glu407) Accession #AAA36770	IEGRID	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ala33

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  67.4 kDa (monomer)

• SDS-PAGE

  115-125 kDa, reducing conditions

Background: TrkA

Trk A, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. Trk A, Trk B and Trk C preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct Trk A isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer Trk A isoform is the only isoform expressed within neuronal tissues whereas the shorter Trk A is expressed mainly in non-neuronal tissues. NGF binds to Trk A with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of Trk A with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily. NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when expressed independent of Trk.

• References:

1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.

2. Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.

• Long Name:

  Neurotrophic Tyrosine Kinase Receptor A

• Entrez Gene IDs:

  4914 (Human); 59109 (Rat)

• Alternate Names:

  DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A