详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its binding ability in a functional ELISA. When recombinant human TLR-4 is Immobilized at 2 µg/mL (100 µL/well), the concentration of rhMD-2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 µg/mL.
- SourceE. coli-derived
Met Human MD-2
(Glu17 - Asn160)
Accession # BAA78717IEGRGGGSGGGSGGGS 10-His tag N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisMet - Predicted Molecular Mass19.2 kDa (monomer)
1787-MD/CF | | 1787-MD |
Formulation Supplied as a 0.2 μm filtered solution in Acetonitrile and TFA. | Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. | |
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. | ||
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | |
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
MD-2, also known as lymphocyte antigen 96 and ESOP-1, is a secreted glycoprotein that shares conserved cysteine residues and significant sequence similarity (23%) with MD-1. The gene of human MD-2 encodes a 160 amino acid residue (aa) precursor protein with a 16 aa signal peptide and a 144 aa mature protein, which contains 2 N‑glycosylation sites (1). Recombinant secreted MD‑2 has been found to exist as disulfide-linked dimers and oligomers (2).
Both MD-1 and MD-2 are accessory molecules that associate with the extracellular leucine-rich repeats (LRR) of Toll-like receptor (TLR) family members, which are type I transmembrane receptors that regulate innate immune responses to microbial pathogens (3, 4). MD-1 binds to RP105 on B cells and macrophages to form the signaling receptor complex for lipopolysaccharide (LPS), a constituent of the outer membrane of Gram-negative bacteria. Similarly, MD-2 interacts with TLR-4 to form the heteromeric receptor that confers LPS responsiveness. MD-2 also associates with TLR-2, albeit with less avidity, to confer responsiveness to cell wall components from both Gram-positive and Gram-negative bacteria. MD-1 and MD-2 are also required for the correct targeting of the TLRs to the cell surface. Although MD-2 glycosylation is not crucial for its surface expression and interaction with TLR-4, it is required for LPS binding and signaling (5).
- References:
- Shimazu, R. et al. (1999) J. Exp. Med. 189:1777.
- Visintin, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:12156.
- Nagai, Y. et al. (2002) Nature Immunology 3:667.
- Akashi, S. et al. (2003) J. Exp. Med. 198:1035.
- Correia, J. and R. Ulevitch (2002) J. Biol. Chem. 277:1845.
- Long Name:Myeloid Differentiation Protein 2/Lymphocyte Antigen 96
- Entrez Gene IDs:23643 (Human)
- Alternate Names:ESOP1; ESOP-1; LY96; ly-96; lymphocyte antigen 96; MD2; MD-2; myeloid differentiation protein-2; Protein MD-2