详细说明
- Purity>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to bind fluorescein-conjugated S. aureus Bioparticles. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834. The ED 50 for this effect is 0.4-2 µg/mL.
- SourceMouse myeloma cell line, NS0-derived
Human LILRB3/CD85a/ILT5
(Gly24-Glu443)
Accession # AAB68668IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisGly24 - Structure / FormDisulfide linked homodimer
- Predicted Molecular Mass72.6 kDa (monomer)
- SDS-PAGE95-105 kDa, reducing conditions
1806-T5 | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 250 μg/mL in PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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85-95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3). Alternative splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors (4-6). Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5, 7). On osteoclast precursors, LILRB3 ligation inhibits RANK L/TRANCE or M-CSF induced differentiation (8). LILRB3 can also bind to ligands exposed on necrotic tumor cells (9). Both PIR-B and LILRB3 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (3). R&D Systems in-house testing indicates that LILRB3 binds to
Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (10). In the mouse CNS, PIR-B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (11). Upon binding to MAG, PIR-B associates with TrkB and NGF R/p75 in cerebellar granule neurons (12).
- References:
- Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
- Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
- Nakayama, M. et al. (2007) J. Immunol. 178:4250.
- Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1174.
- Sloane, D.E. et al. (2004) Blood 104:2832.
- Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
- Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
- Mori, Y. et al. (2008) J. Immunol. 181:4742.
- Jones, D.C. et al. (2016) Oncotarget PMID 26769854.
- Zheng, J. et al. (2012) Nature 485:656.
- Atwal, J.K. et al. (2008) Science 322:967.
- Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.
- Entrez Gene IDs:11025 (Human)
- Alternate Names:CD85 antigen-like family member A; CD85a antigen; CD85a; HL9Immunoglobulin-like transcript 5; ILT5; ILT-5; ILT5CD85a; Leukocyte immunoglobulin-like receptor 3; leukocyte immunoglobulin-like receptor subfamily B member 3; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB3; LIR3; LIR3CD85A; LIR-3MGC138403; member 3; Monocyte inhibitory receptor HL9; PIRB