• Purity

  >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. Bechard, D.     et al. (2001) J. Immunol.     167:3099.

  When 1 x 10⁵ cells/well are added to Recombinant Human Endocan/ESM coated plates, adhesion is induced in a dose dependent manner after a 1 hour incubation at 37 °C. The ED₅₀ for this effect is 2-10 μg/mL.

  
  

  Optimal dilutions should be determined by each laboratory for each application.

• Source

  Mouse myeloma cell line, NS0-derived Trp20-Arg184, with a C-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Trp20

• Predicted Molecular Mass

  19.5 kDa

• SDS-PAGE

  24-27 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Endocan/ESM-1

Endocan, also known as endothelial-cell specific molecule-1 (ESM-1), is a secreted cysteine-rich dermatan sulfate (DS) proteoglycan primarily expressed by endothelial cells within the vascular capillary network in kidney and in the alveolar walls of the lung (1). Endocan expression has also been detected in different epithelia and in adipocytes (2, 3). The expression of endocan is upregulated by TNF alpha, IL-1 beta or lipopolysaccharide and down-regulated by IFN gamma (1). The human Endocan gene encodes a 184 amino acid (aa) residues precursor protein with a 19 aa hydrophobic signal peptide and a 165 aa mature region with 18 Cysteine residues (1). The DS chain is covalently attached to serine 137 (4). Endocan has been shown to bind CD11a/CD18 integrin (also known as lymphocyte function-associated antigen-1, LFA-1) on human lymphocytes, monocytes and Jurkat cells, inhibiting its binding to ICAM-1 and reducing LFA-1-mediated leukocyte activation (5). Endocan binds via its DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity (3, 6). Genetically engineered cells overexpressing endocan has been shown to induce tumor formation, suggesting that Endocan may be involved in the pathophysiology of tumor growth in vivo (3, 6). Circulating Endocan can be detected in the serum from healthy subjects. In patients with lung cancer or acute and severe sepsis, elevated Endocan concentrations have been reported (2, 6).

• References:

1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.

2. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.

3. Wellner, M., et al. (2003) Horm. Metab. Res. 35:217.

4. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.

5. Bechard, D. et al. (2001) J. Immunol. 167:3099

6. Scherpereel, A. et al. (2003) Cancer Res. 63:6084.

• Entrez Gene IDs:

  11082 (Human); 71690 (Mouse)

• Alternate Names:

  Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6