• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells. The ED    ₅₀ for this effect is 0.5-2.0 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Human AMIGO2 (Gly39-His393) Accession # Q86SJ2	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Gly39 & Val40

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  66.9 kDa (monomer)

• SDS-PAGE

  90-110 kDa, reducing conditions

Background: AMIGO2

AMIGO2 (amphoterin‑induced gene and ORF 2), also known as DEGA and Alivin‑1, is an approximately 65 kDa transmembrane cell adhesion protein. It belongs to a family of leucine‑rich repeat (LRR) containing proteins that play various roles in nervous system development and function (1, 2). Mature human AMIGO2 consists of a 359 amino acid (aa) extracellular domain (ECD) with six LRRs flanked by single LRRNT and LRRCT domains, followed by one immunoglobulin-like domain, a 21 aa transmembrane segment, and a 103 aa cytoplasmic domain (3‑5). Within the ECD, human AMIGO2 shares 89% and 87% aa sequence identity with mouse and rat AMIGO2, respectively. AMIGO2 forms homodimers as well as heterodimers with the related AMIGO and AMIGO3 molecules (3, 6). Within the nervous system, AMIGO2 is transcribed in the cerebrum, hypothalamus, olfactory bulb, retina, hippocampus (pyramidal cells), and cerebellum (granule neurons and Purkinje cells) (3, 4, 7). It is also expressed in the liver, lung, testis, spleen, and small intestine (3, 4). AMIGO2 supports neuron survival, and is down‑regulated following pro‑apoptotic stimulation (4). Its expression can be up‑regulated or down‑regulated in a variety of cancers, and anti‑sense knockdown of AMIGO2 interferes with tumor cell adhesion to collagen as well as   in vivo tumorigenicity (5).

• References:

1. de Wit, J. et al. (2011) Annu. Rev. Cell Dev. Biol. 27:697.

2. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.

3. Kuja-Panula, J. et al. (2003) J. Cell Biol. 160:963.

4. Ono, T. et al. (2003) J. Neurosci. 23:5887.

5. Rabenau, K.E. et al. (2004) Oncogene 23:5056.

6. Kajander, T. et al. (2011) J. Mol. Biol. 413:1001.

7. Laeremans, A. et al. (2013) Brain Struct. Funct. 218:123.

• Long Name:

  Amphoterin-induced Gene and ORF 2

• Entrez Gene IDs:

  347902 (Human); 105827 (Mouse)

• Alternate Names:

  adhesion molecule with Ig-like domain 2; ALI1amphoterin induced gene 2; alivin 1; Alivin-1; AMIGO2; AMIGO-2; DEGAamphoterin-induced protein 2; differentially expressed in gastric adenocarcinoma; Differentially expressed in gastric adenocarcinomas; transmembrane protein AMIGO2