详细说明
- Purity>95%, by SDS-PAGE with silver staining.
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED 50 for this effect is 0.15-0.75 μg/mL.
- SourceE. coli-derived Gln23-Asn166, with an N-terminal Met
- Accession #
- N-terminal Sequence
AnalysisMet - Predicted Molecular Mass16 kDa
- SDS-PAGE16 kDa, reducing conditions
| 2090-RG | | |
| Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
| Reconstitution Reconstitute at 500 μg/mL in PBS. | ||
| Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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| Bioactivity | Recombinant Human Reg1B (Catalog # 2090-RG) stimulates cell proliferation in RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.15-0.75 μg/mL. |
| SDS-PAGE | 1 μg/lane ofRecombinant Human Reg1B (Catalog # 2090-RG)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing bands at 17.2 and 16.7 kDa, respectively. |
Reg1B also called Lithostatine-1-beta or Pancreatic stone protein 2 (PSP2) is a type I subclass member of the Reg gene family. The Reg multigene family consists of four subclasses, types I, II, III and IV based on the primary structures of the encoded proteins of the genes. Reg1B is also a member of the Reg family of secreted C-type lectin domain-containing pancreatic proteins (1, 2). The human Reg1B cDNA encodes 166 amino acids protein which contains a 22 aa signal peptide and the 144 aa secreted mature protein. Reg1B shares 86% animo acide sequence identity with Reg1A and like Reg1A is secreted by the exocrine pancreas and associated with islet cell regeneration and diabetogenesis. Reg family proteins were also shown to cause Schwann cell proliferation during regeneration of motor neurons (3).
- References:
- Liu, J.-L. et al. (2008) Endocrine Metab. Immune Disord. Drug Targets 8:1.
- Unno, M. et al. (1993) J. Biol. Chem. 268:15974.
- Livesey F.J. et al. (1990) Nature. 390:614.
- Long Name:Regenerating Islet-derived 1B
- Entrez Gene IDs:5968 (Human)
- Alternate Names:Lithostathine 1 beta; lithostathine-1-beta; Pancreatic stone protein 2; pancreatic threadprotein); PSPS2; PSPS2PSP-2; Reg1B; regenerating islet-derived 1 beta; Regenerating islet-derived protein 1-beta; Regenerating protein I beta; REGH; REGLREG-1-beta; secretory pancreatic stone protein 2
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