详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
- Endotoxin Level<0.10 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 2.5-5.0 µg/mL of Recombinant Human Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta ‑NGF (Catalog # ).
Optimal dilutions should be determined by each laboratory for each application. - SourceMouse myeloma cell line, NS0-derived
Human Semaphorin 6B
(Leu26-Ser603)
Accession # Q9H3T3IEGRMD Human IgG1
(Pro100-Lys330)N-terminus C-terminus - Accession #
- N-terminal Sequence
AnalysisLeu26 - Structure / FormDisulfide-linked homodimer
- Predicted Molecular Mass63.4 kDa (monomer)
- SDS-PAGE110-120 kDa, reducing conditions
2094-S6 | | |
Formulation Lyophilized from a 0.2 μm filtered solution in PBS. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- References:
- Pasterkamp, R.J. and R.J. Giger (2009) Curr. Opin. Neurobiol. 19:263.
- Neufeld, G. et al. (2005) Front. Biosci. 10:751.
- Roney, K. et al. (2013) Protein Cell 4:17.
- Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
- Correa, R.G. et al. (2001) Genomics 73:343.
- Murad, H. et al. (2006) Int. J. Oncol. 28:977.
- Ge, C. et al. (2013) J. Int. Med. Res. 41:284.
- Collet, P. et al. (2004) Genomics 83:1141.
- Murad, H. et al. (2011) Mol. Med. Rep. 4:575.
- Suto, F. et al. (2005) J. Neurosci. 25:3628.
- Tawarayama, H. et al. (2010) J. Neurosci. 30:7049.
- Entrez Gene IDs:10501 (Human); 20359 (Mouse)
- Alternate Names:sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; Sema6B; SEMAN; Semaphorin 6B; semaphorin VIB; semaphorin Z; semaphorin-6B; semaphorin-6Ba; SEMA-VIB; SEMAZ; SEM-SEMA-Y; UNQ1907/PRO4353