• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.01 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind biotinylated N-Acetylglucosamine-Polyacrylamide in a functional ELISA.

• Source

  Mouse myeloma cell line, NS0-derived Leu26-Ala313, with a C-terminal 10-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Leu26

• Predicted Molecular Mass

  32.8 kDa

• SDS-PAGE

  39-42 kDa, reducing conditions

Background: Ficolin-2

Human Ficolin-2 (fibrinogen/collagen-like; previously called L-ficolin or ficolin-B) is a member of the ficolin family of secreted pattern recognition proteins that participate in the lectin complement activation pathway (1 - 4). Ficolin-2 is expressed in the liver and released into the circulation (2). The 35 - 40 kDa, 313 amino acid (aa) human Ficolin-2 contains a 25 aa signal sequence, an N-terminal collagen domain and a C-terminal fibrinogen-like domain that includes a calcium binding site and two potential N-glycosylation sites. The collagen domain mediates trimer formation. Larger homo-multimers are formed by disulfide links at the N-terminus, the most prominent of which is a 12 subunit oligomer (3, 5). Ficolin-2 binds microbial ligands that contain acetylated compounds (6). Notably, this includes N-acetyl glucosamine in compounds such as lipoteichoic acid in gram-positive bacteria. It also binds fungal 1,3-beta -D-glucan (4, 7, 8). Pathogen recognition by Ficolin-2 initiates an immune response that involves calcium-dependent interaction of Ficolin-2 with the MBL-associated serine protease (MASP) complex. This complex cleaves C4 to activate the complement pathway (4, 7, 8). In a secondary role, Ficolin-2 is known to bind late apoptotic and necrotic cells, probably through the recognition of exposed DNA. This also activates the complement cascade that assists in clearance of cells (9, 10). Mature human Ficolin-2 shares 70%, 72%, 76% and 78% aa identity with mouse, rat, cow and pig Ficolin-2, respectively. It shares 84% and 52% aa identity with human ficolin-1 and ficolin-3, respectively. Single nucleotide polymorphisms are common in human Ficolin-2. Some affect serum concentration, while others can increase or decrease ligand binding (11).

• References:

1. Endo, Y. et al. (2006) Adv. Exp. Med. Biol. 586:265.

2. Endo, Y. et al. (1996) Genomics 36:515.

3. Matsushita, M. et al. (1996) J. Biol. Chem. 271:2448.

4. Ma, Y. G. et al. (2004) J. Biol. Chem. 279:25307.

5. Hummelshoj, T. et al. (2007) Mol. Immunol. 44:401.

6. Krarup, A. et al. (2004) J. Biol. Chem. 279:47513.

7. Garlatti, V. et al. (2007) EMBO J. 26:623.

8. Lynch, N. J. et al. (2004) J. Immunol. 172:1198.

9. Jensen, M. L. et al. (2007) Mol. Immunol. 44:856.

10. Kuraya, M. et al. (2005) Immunobiology 209:689.

11. Hummelshoj, T. et al. (2005) Hum. Mol. Genet. 14:1651.

• Long Name:

  Ficolin [Collagen/Fibrinogen Domain Containing] 2

• Entrez Gene IDs:

  2220 (Human)

• Alternate Names:

  37 kDa elastin-binding protein; Collagen/fibrinogen domain-containing protein 2; EBP-37ficolin (collagen/fibrinogen domain-containing lectin) 2 (hucolin); FCN2; FCNL; FCNLficolin B; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin); Ficolin2; Ficolin-2; ficolin-B; ficolin-beta; Hucolin; L-Ficolin; P35; Serum lectin p35