• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 μL droplet containing 90 ng on a nitrocellulose coated microplate.

• Source

  Mouse myeloma cell line, NS0-derived Ser1583-Ser2224, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ser1583

• Predicted Molecular Mass

  69 kDa

• SDS-PAGE

  100-110 kDa, reducing conditions

Background: NG2/MCSP

NG2, also known as CSPG4, MCSP, and AN2, is a 400-500 kDa transmembrane chondroitin sulfate proteoglycan (CSPG) with a protein core of approximately 300 kDa. The extracellular region can be proteolytically shed from the cell surface (1-3). Mature human NG2 consists of a 2195 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (4). Within aa 1583-2224, human NG2/CSPG4 shares 83% aa sequence identity with mouse and rat CSPG4. NG2 binds to the extracellular matrix proteins Laminin, Tenascin, and Collagens II, V, and VI as well as to the growth factors FGF-2 and PDGF-AA (1, 5, 6). NG2 is expressed on glial cell progenitors known as O2A cells or NG2 glia (2, 7, 8). These cells are neuronally responsive and differentiate primarily into oligodendrocytes but also into astrocytes (8-10). NG2 associates with PDGF R alpha and the AMPA R subunit GluR2 (2, 7). It is up-regulated on microglial cells during inflammation and contributes to the induction of inflammatory mediators (11). Various CSPGs in the brain inhibit neurite outgrowth through interactions with Nogo Receptor/NgR1 and NgR3 (12). This recombinant protein product corresponds to the last 5 CSPG repeats, a region which can independently inhibit neurite outgrowth (13). NG2 is also expressed on vascular mural cells and capillaries (10, 14). It promotes vascular endothelial cell (EC) migration and angiogenesis through interactions with Galectin-3 and Integrin alpha 3 beta 1 on EC, Plasminogen, and Angiostatin (15, 16). NG2 is also expressed on a variety of tumors where it contributes to tumor cell adhesion, motility, and invasion (17).

• References:

1. Campoli, M. et al. (2010) Adv. Cancer Res. 109:73.

2. Nishiyama, A. et al. (1996) J. Neurosci. Res. 43:315.

3. Nishiyama, A. et al. (1995) Mol. Biol. Cell 6:1819.

4. Pluschke, G. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9710.

5. Burg, M.A. et al. (1996) J. Biol. Chem. 271:26110

6. Goretzki, L. et al. (1999) J. Biol. Chem. 274:16831.

7. Stegmuller, J. et al. (2003) J. Biol. Chem. 278:3590.

8. Komitova, M. et al. (2009) J. Comp. Neurol. 512:702.

9. Bergles, D.E. et al. (2000) Nature 405:187.

10. Zhu, X. et al. (2008) Development 135:145.

11. Gao, Q. et al. (2010) Neuroscience 165:386.

12. Dickendesher, T.L. et al. (2012) Nat. Neurosci. 15:703.

13. Ughrin, Y.M. et al. (2003) J. Neurosci. 23:175.

14. Schlingemann, R.O. et al. (1990) Am. J. Pathol. 136:1393.

15. Fukushi, J. et al. (2004) Mol. Biol. Cell 15:3580.

16. Goretzki, L. et al. (2000) J. Biol. Chem. 275:28625.

17. Wang, X. et al. (2010) Curr. Mol. Med. 10:419.

• Long Name:

  Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

• Entrez Gene IDs:

  1464 (Human); 121021 (Mouse); 81651 (Rat)

• Alternate Names:

  AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8