• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. Immobilized rhGlypican 5 at 5 µg/mL (100 µL/well) can bind rhFGF-basic with a linear range of 0.16-10 ng/mL.

• Source

  Mouse myeloma cell line, NS0-derived Glu25-Thr554, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Glu25

• Predicted Molecular Mass

  59.9 kDa (monomer)

• SDS-PAGE

  61-67 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Glypican 5

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked proteoglycans that likely play a key role in embryonic morphogenesis (1 - 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60 - 70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of glypicans are known, with one subfamily containing Glypicans-1, 2, 4 and 6, and another subfamily containing Glypicans-3 and 5 (3, 5). Human Glypican-5 (GPC-5) is synthesized as a 572 amino acid (aa) preproprecursor that contains a 24 aa signal sequence, a 532 aa mature region and a 16 aa C-terminal prosegment (6, 7). There are three potential N-linked, and five potential O-linked sites for glycosylation or glycanation. GPC-5 is believed to contain 6 - 7 kDa of glycosylation and at least 55 kDa of proteoglycan. This is based on an assumption of the presence of one heparan sulfate chain of 36 kDa and one chrondroitin sulfate chain of 17 kDa (7, 8). When added to the core molecular weight of 59 kDa, the mature protein is approximately 120 kDa in size. To date, however, the actual size of native human GPC-5 has not been reported and the suggestion of a chrondroitin sulfate modification is based on the expression of human GPC-5 in COS-7 cells (7). Human to mouse, there is 88% aa identity over the mature region. Cells known to express GPC-5 are principally embryonic in nature, and include neurons and mesenchyme (1, 7). The function of GPC-5 is essentially unknown. As a glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (9, 10).

• References:

1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241.

2. Filmus, J. (2001) Glycobiology 11:19R.

3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117.

4. Filmus, J. and S.B. Selleck (2001) J. Clin. Invest.108:497.

5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.

6. Veugelers, M. et al. (1997) Genomics 40:24.

7. Saunders, S. et al. (1997) Dev. Biol. 19:78.

8. Rapraeger, A. et al. (1985) J. Biol. Chem. 260:11046.

9. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.

10. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.

• Entrez Gene IDs:

  2262 (Human); 103978 (Mouse)

• Alternate Names:

  bA93M14.1; Glypican 5; glypican proteoglycan 5; glypican-5; GPC5