Recombinant Human Biglycan Protein, CF 50 UG

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Recombinant Human Biglycan Protein, CF 50 UG信息二维码

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产品介绍

    基本参数

    详细说明

    • Purity

      >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

    • Endotoxin Level

      <1.0 EU per 1 μg of the protein by the LAL method.  

    • Activity

      Measured by its ability to inhibit the cell growth of mouse 3T3‑L1 mouse embryonic fibroblast adipose-like cells. Ward, M. and Ajuwon, K.M. (2011) Cell Proliferation     44:343. The ED    50 for this effect is 1.5-6 μg/mL. Measured by its ability to increase the binding of BMP-4 to Chordin. Moreno, M.     et al. (2005) EMBO J.     24:1397. 2-8 µg/mL of Recombinant Human Biglycan  will have a 50% maximum increase in the binding of recombinant human BMP-4 (0.1 µg/mL) to recombinant mouse Chordin (1 µg/mL).

    • Source

      Mouse myeloma cell line, NS0-derived Asp38-Lys368, with a C-terminal 6-His tag

    • Accession #

    • N-terminal Sequence    
      Analysis

      Asp38

    • Predicted Molecular Mass

      38 kDa

    • SDS-PAGE

      40-45 kDa, reducing conditions

    2667-CM

     

    Formulation Lyophilized from a 0.2 μm filtered solution in PBS.


    Reconstitution Reconstitute at 100 μg/mL in sterile PBS.



    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.


    Stability & Storage:       Use a manual defrost freezer and avoid repeated freeze-thaw cycles.      

    • 12 months from date of receipt, -20 to -70 °C as supplied.

    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.


    Background: Biglycan

    Biglycan, also known as PG I, is a secreted chondroitin/dermatan sulfate proteoglycan in the small leucine-rich proteoglycan (SLRP) family. SLRP family members are characterized by N-terminal and C-terminal cysteine-rich domains that flank the central region containing 10-12 tandem leucine-rich repeats (LRRs). Biglycan function is important in the development and maintenance of many tissues (1). The human Biglycan cDNA encodes a 368 amino acid (aa) precursor with a 19 aa signal sequence and a 18 aa propeptide that is cleaved by BMP-1 (2). Mature human Biglycan shares 97% aa sequence identity with bovine, mouse, and rat Biglycan. The mature protein can be further cleaved by proteases, which compromises its inability to bind to other molecules (3, 4). The 45 kDa core protein is approximately one third the molecular weight of the fully glycanated form and can assemble into noncovalently-associated dimers (5). Biglycan binds, crosslinks, and stabilizes several proteins in the collagen matrix (6). It also binds and modulates the activity of a variety of non-matrix proteins, including C1q, collectins, TGF-beta, and Wnt3a (3, 7, 8). Biglycan binds to TLR2, TLR4, P2X4, and P2X7, leading to the increased production of inflammatory mediators, the migration of vascular endothelial cells, and the enhancement of oxidized LDL induced aortic valve calcification (9 - 11). Biglycan also blocks the uptake and degradation of acetylated LDL in atherosclerotic plaques by binding to SR-A (12). It stabilizes the neuromuscular junction through interactions with MuSK as well as alpha- and gamma-Sarcoglycan (13).

    • References:

        1. Nastase, M.V. et al. (2012) J. Histochem. Cytochem. 60:963.

        2. Scott, I.C. et al. (2000) J. Biol. Chem. 275:30504.

        3. Boivin, W.A. et al. (2012) PLoS ONE 7:e33163.

        4. Monfort, J. et al. (2006) Arthritis Res. Ther. 8:R26.

        5. Scott, P.G. et al. (2006) J. Biol. Chem. 281:13324.

        6. Wiberg, C. et al. (2003) J. Biol. Chem. 278:37698.

        7. Groeneveld, T.W.L. et al. (2005) J. Immunol. 175:4715.

        8. Berendsen, A.D. et al. (2011) Proc. Natl. Acad. Sci. USA 108:17022.

        9. Babelova, A. et al. (2009) J. Biol. Chem. 284:24035.

        10. Yamamoto, K. et al. (2012) Br. J. Cancer 106:1214.

        11. Song, R. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:2711.

        12. Santiago-Garcia, J. et al. (2003) J. Biol. Chem. 278:6942.

        13. Amenta, A.R. et al. (2012) J. Neurosci. 32:2324.

      1. Entrez Gene IDs:

        633 (Human); 12111 (Mouse)

      2. Alternate Names:

        BGN; Biglycan; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; PGI; PG-S1; SLRR1A; SLRR1Abiglycan proteoglycan; small leucine-rich protein 1A








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