详细说明
- Purity>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
- Endotoxin Level<1.0 EU per 1 μg of the protein by the LAL method.
- ActivityMeasured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 10 4 cells/well are added to Recombinant Human MFG‑E8 coated plates (10 µg/mL, 100 µL/well), approximately 45‑75% will adhere after 60 minutes at 37 °C.
- SourceMouse myeloma cell line, NS0-derived Leu24-Cys387, with a C-terminal 6-His tag
- Accession #
- N-terminal Sequence
AnalysisLeu24 - Predicted Molecular Mass41.6 kDa
- SDS-PAGE45-50 kDa, reducing conditions
2767-MF | | |
Formulation Lyophilized from a 0.2 μm filtered solution in NaH 2PO 4 and NaCl. | ||
Reconstitution Reconstitute at 100 μg/mL in sterile PBS. | ||
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. | ||
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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- References:
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- Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.
- Long Name:Milk Fat Globule EGF Factor 8
- Entrez Gene IDs:4240 (Human); 17304 (Mouse)
- Alternate Names:BA46; Breast epithelial antigen BA46; EDIL1; hP47; HsT19888; lactadherin; Lactahedrin; Medin; MFG1; MFGE8; MFG-E8; MFGM; milk fat globule-EGF factor 8 protein; Milk fat globule-EGF factor 8; O-acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; sperm associated antigen 10; sperm surface protein hP47