• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells. The ED    ₅₀ for this effect is 0.15-0.75 μg/mL.

• Source

  E. coli-derived Phe21-Gly104

• Accession #

• N-terminal Sequence    
  Analysis

  Phe21

• Predicted Molecular Mass

  8.2 kDa

• SDS-PAGE

  6 kDa, reducing conditions

Background: HIN-1/SCGB3A1

High in Normal‑1 (HIN‑1), also known as Uteroglobin related protein 2 (UGRP2), is an 8 kDa secreted protein of the secretoglobin superfamily (SCGB3A1) (1). HIN‑1 is expressed in bronchial epithelial and secretory Clara cells, mammary epithelial cells (particularly during pregnancy), salivary glands, and the prostate (2‑5). It is up‑regulated in Clara cells by IL‑4, IL‑13, Oncostatin M, EGF, and TGF‑ alpha and down‑regulated by IFN‑ alpha, beta, and gamma (6‑9). It is secreted into bronchial lavage fluid, saliva, and plasma, and may form disulfide‑linked dimers (10). HIN‑1 binds to the macrophage scavenger receptor MARCO, and to the surface of mammary and bronchial epithelial cells (11, 12). HIN‑1 promotes apoptosis and inhibits the proliferation, migration, and invasion of breast cancer cells (12). The down‑regulation of HIN‑1 expression in many breast, lung, and prostate cancers correlates with hypermethylation of its promoter (4, 10, 13, 14). Mature human HIN‑1 shares 58% and 62% aa sequence identity with mouse and rat HIN‑1, respectively (4).

• References:

1. Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.

2. Porter, D. et al. (2002) Mech. Dev. 114:201.

3. Reynolds, S.D. et al. (2002) Am. J. Respir. Crit. Care Med. 166:1498.

4. Krop, I. et al. (2001) Proc. Natl. Acad. Sci. 98:9796.

5. Niimi, T. et al. (2002) Cytogenet. Genome Res. 97:120.

6. Yamada, A. et al. (2005) J. Immunol. 175:5708.

7. Tomita, T. et al. (2009) Am. J. Respir. Cell Mol. Biol. 40:620.

8. Yamada, A. and S. Kimura (2005) FEBS Lett. 579:2221.

9. Yamada, A. et al. (2009) Biochem. Biophys. Res. Commun. 379:964.

10. Krop, I. et al. (2004) Mol. Cancer Res. 2:489.

11. Bin, L.-H. et al. (2003) J. Immunol. 171:924.

12. Krop, I. et al. (2005) Cancer Res. 65:9659.

13. Krop, I. et al. (2003) Cancer Res. 63:2024.

14. Shigematsu, H. et al. (2005) Int. J. Cancer 113:600.

• Long Name:

  High in Normal 1

• Entrez Gene IDs:

  92304 (Human); 73945 (Mouse)

• Alternate Names:

  cytokine high in normal-1; Cytokine HIN-1; High in normal 1; HIN1; HIN-1; HIN1Pneumo secretory protein 2; LU105; PNSP2; PnSP-2; PnSP-2Uteroglobin-related protein 2; SCGB3A1; secretoglobin family 3A member 1; secretoglobin, family 3A, member 1; UGRP2; UGRP2MGC87867