• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind biotinylated Neu5Ac alpha 2-6GalNAc alpha (sialyl-Tn). Patel, N.     et al. (1999) The Journal of Biological Chemistry     274:22729.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Human Siglec-6 (Gln27 - Val331) Accession # NP_942142	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln27 predicted

• Predicted Molecular Mass

  60.3 kDa (monomer)

• SDS-PAGE

  80-95 kDa, reducing conditions

Background: Siglec-6/CD327

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins that belong to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains (1 - 4). Eleven human Siglecs (Siglec-1 through 11) have been cloned and characterized. Within these eleven, there are at least two groups, one of which is termed the CD33-related group. CD33-related Siglecs include CD33/Siglec-3 and Siglec-5 through 11 (1, 3). To date, no Siglec has been shown to recognize any cell surface ligand other than sialic acid. This suggests that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. The cDNA of human Siglec-6 (also known as OB-BP1 and CD33L), encodes a putative 442 amino acid (aa) protein that contains a 15 aa signal peptide, a 321 aa extracellular region, a 21 aa transmembrane region (TM), and an 85 aa cytoplasmic tail (5, 6). The extracellular region contains one N-terminal V-type Ig-like domain followed by two Ig-like C2-type domains. The cytoplasmic domain has one immunoreceptor tyrosine-based inhibition motif (ITIM). At least three additional isoforms exist, all of which encode an additional 11 aa’s at the N-terminus, likely due to the utilization of an alternate start site. Two of the three isoforms also show splicing. One isoform shows a 16 aa in-frame deletion in the second C2-like domain, while the other shows a deletion of the TM and cytoplasmic region, thus potentially generating a soluble form (6 - 9). Siglec-6 is found on B cells and in placenta, and would seem to have a restricted specificity for the sialyl Tn antigen (6, 10).

• References:

1. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.

2. Crocker, P.R. and A. Varki (2001) Immunology 103:137.

3. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.

4. Powell, L.D. and A. Varki (1995) J. Biol. Chem. 270:14243.

5. Takei, Y. et al. (1997) Cytogenet. Cell Genet. 78:295.

6. Patel, N. et al. (1999) J. Biol. Chem. 274:22729.

7. GenBank Accession # NP_942143.

8. GenBank Accession #: NP_942142.

9. GenBank Accession #: NP_001236.

10. Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.

• Long Name:

  Sialic Acid Binding Ig-like Lectin 6

• Entrez Gene IDs:

  946 (Human)

• Alternate Names:

  CD327 antigen; CD327; CD33 antigen-like 1; CD33L1; CD33L1OBBP1CD327; CD33L2; CD33LCDW327; CDw327; OB-BP1; Obesity-binding protein 1; sialic acid binding Ig-like lectin 6; sialic acid-binding Ig-like lectin 6; Siglec6; Siglec-6