• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. When 5 x 10    ⁴ cells/well are added to rhFLRT2 coated plates (5 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 30 minutes at 37° C.    
     Optimal dilutions should be determined by each laboratory for each application.  

• Source

  Mouse myeloma cell line, NS0-derived Cys36-Ser539, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Cys36

• Predicted Molecular Mass

  57.1 kDa

• SDS-PAGE

  75-85 kDa, reducing conditions

Background: FLRT2

FLRT2 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85 kDa type I transmembrane (TM) human FLRT2 is synthesized as a 660 amino acid (aa) precursor with a 35 aa signal sequence, a 506 aa extracellular domain (ECD), a 21 aa TM segment and a 98 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The human FLRT2 ECD shares 97%, 96%, 99%, 96% and 95% aa sequence identity with mouse, rat, equine, canine and bovine FLRT2 ECD, respectively. Human FLRT1 and FLRT3 ECDs share approximately 47% aa identity with FLRT2. The fibronectin domain of all three FLRTs can bind to FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). In adulthood, FLRT2 mRNA is most abundant in pancreas, but is also present in skeletal muscle, brain and heart (1). FLRT2 in mouse embryos shows highest expression in a subset of the sclerotome in the brain, the stomach, and posterior to the developing heart (2). This expression is distinct from that of FLRT1 and FLRT3 (2).

• References:

1. Lacy, S. E. et al. (1999) Genomics 62:417.

2. Haines, B. P. et al. (2006) Dev. Biol. 297:14.

3. Bottcher, R. T. et al. (2004) Nat. Cell Biol. 6:38.

4. Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.

5. Ogata, S. et al. (2007) Genes Dev. 21:1817.

• Long Name:

  Fibronectin Leucine Rich Transmembrane Protein 2

• Entrez Gene IDs:

  23768 (Human)

• Alternate Names:

  Fibronectin Leucine Rich Transmembrane Protein 2; Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 2; FLRT2; KIAA0405; Leucine-Rich Repeat Transmembrane Protein FLRT2