• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. When rhNectin-3 is coated at 1 μg/mL, rhNectin-1 binds with an apparent K    _D <25 nM.  

• Source

  Mouse myeloma cell line, NS0-derived Gln31-Thr334 with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  No results obtained: Gln31 predicted

• Predicted Molecular Mass

  35.0 kDa

• SDS-PAGE

  50-65 kDa, reducing conditions

Background: Nectin-1

Nectin-1 (designated CD111), also called PRR-1 (poliovirus receptor-related protein 1) or HVEC (herpesvirus entry mediator C), is a widely expressed 110 kDa type I transmembrane glycoprotein important in formation of adherens junctions and synapses. It is a member of the nectin family within the Ig superfamily (1, 2). The Latin word necto means “to connect”, indicating the role of nectins in Ca²⁺-independent cell-cell adhesion (2). Nectin-1 forms homodimers in cis, followed by interactions in trans with Nectin-1, -3 or -4 (2). The 517 amino acid (aa) human Nectin-1 isoform 1 contains a 30 aa signal sequence, a 325 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 141 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N-terminal V-type that mediates ligand binding, and two C2-type (3). Nectin-1, like other nectins, has a splice form (isoform 2 or HigR, 458 aa) with alternate TM and cytoplasmic sequences. Another, isoform 3, is a 352 aa secreted protein (4). The common region of mature human Nectin-1 (aa 31 - 334) shares 93%, 94%, 96% and 96% aa identity with mouse, rat, bovine and porcine Nectin-1, respectively. Nectin-1 binds viral glycoprotein D to mediate herpesvirus (but not poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts (4 - 7). In forming adherens junctions and synapses, nectins 1 and 3 initiate cell-cell interactions, recruiting alpha _v beta ₃ integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins (2, 8 - 11). These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 also recognizes CD96 on NK cells (12). Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia (13). Nectin-1 downregulation in epithelial cancers, mediated in part by ectodomain shedding, may contribute to invasiveness (14).

• References:

1. Lopez, M. et al. (1995) Gene 155:261.

2. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.

3. Fabre, S. et al. (2002) J. Biol. Chem. 277:27006.

4. Lopez, M. et al. (2001) J. Virol. 75:5684.

5. Cocchi, F. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15700.

6. Linehan, M. M. et al. (2004) J. Virol. 78:2530.

7. Simpson, S. A. et al. (2005) J. Neurovirol. 11:208.

8. Mizoguchi, A. et al. (2002) J. Cell Biol. 156:555.

9. Togashi, H. et al. (2006) J. Cell Biol. 174:141.

10. Tachibana, K. et al. (2000) J. Cell Biol. 150:1161.

11. Takai, Y. and H. Nakanishi (2003) J. Cell Science 116:17.

12. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.

13. Suzuki, K. et al. (2000) Nat. Genet. 25:427.

14. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.

• Long Name:

  Poliovirus Receptor Related 1

• Entrez Gene IDs:

  5818 (Human)

• Alternate Names:

  CD111 antigen; CD111; CLPED1ectodermal dysplasia 4 (Margarita Island type); ED4; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgRPVRR; HVEC; HVECpoliovirus receptor-like 1; MGC142031; Nectin1; Nectin-1; OFC7nectin 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); poliovirus receptor-related protein 1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; PVRR1nectin-1; SK-12