• Purity

  >95%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED    ₅₀ for this effect is 5-30 ng/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived Ser234-Ile366 ( alpha chain) & Gly311-Ser426 ( beta chain)

• Accession #

• N-terminal Sequence    
  Analysis

  Ser234 ( alpha chain) & Gly311 ( beta chain)

• Structure / Form

  Disulfide-linked heterodimer

• Predicted Molecular Mass

  14.5 kDa ( alpha chain) & 13.0 kDa ( beta chain)

• SDS-PAGE

  12-14 kDa & 17-20 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Data Images

Background: Inhibin A

Inhibin A is a member of the TGF-beta superfamily of proteins (1). Mature Inhibin A is a disulfide-linked dimer composed of alpha and beta subunits (1). Inhibin A shares a common alpha subunit with the closely related protein Inhibin B but has a unique beta subunit ( beta A) (2-4). The mature alpha subunit of mouse Inhibin A has a predicted molecular weight of 14.7 kDa and shares 80% and 97% amino acid (aa) sequence identity with the human and rat orthologs, respectively. The mature beta subunit of this mouse protein has a predicted molecular weight of 13 kDa and shares 93% and 100% aa sequence identity with the human and rat orthologs, respectively. Inhibin binds and antagonizes ActRIIA and ActRIIB in complex with the TGF-beta RIII and/or IGSF1 co-receptors and subsequently acts to suppress Activin-induced Follicle Stimulating Hormone (FSH) secretion (1, 5-7). Inhibins are produced by gonadal cells in both males and females (8). They are thought to be involved in the regulation of gametogenesis, and embryonic and fetal development (8, 9). Elevated concentrations of Inhibins are associated with pregnancy, preeclampsia, and ovarian cancer, and Inhibin A levels are typically measured during prenatal screening for Down’s syndrome (10-14).

• References:

1. Phillips, D.J. and T.K. Woodruff (2004) Growth Factors 22:13.

2. Ling, N. et al. (1985) Proc. Natl. Acad. Sci. USA 82:7217.

3. Robertson, D.M. et al. (1985) Biochem. Biophys. Res. Commun. 126:220.

4. Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957.

5. Lewis, K.A. et al. (2000) Nature 404:411.

6. Martens, J.W. et al. (1997) Endocrinology 138:2928.

7. Chapman, S.C. et al. (2002) Mol. Cell. Endocrinol. 196:79.

8. de Kretser, D.M. and D.M. Robertson (1989) Biol. Reprod. 40:33.

9. Knight, P.G. et al. (2012) Mol. Cell. Endocrinol. 359:53.

10. Walentowicz, P. et al. (2014) PLoS One 9:e90575.

11. Kondi-Pafiti, A. et al. (2013) Clin. Exp. Obstet. Gynecol. 40:109.

12. Kuijper, E.A. et al. (2013) Reprod. Biomed. Online 27:33.

13. Carty, D.M. et al. (2008) Trends Cardiovasc. Med. 18:186.

14. Wald, N.J. et al. (1996) Prenat. Diagn. 16:143.

• Entrez Gene IDs:

  3623 (Human); 16322 (Mouse)

• Alternate Names:

  A-inhibin subunit; INHA; Inhibin A; inhibin alpha chain; inhibin, alpha