• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to bind fluorescein-conjugated     S. aureus Bioparticles. Jiang, Y.     et al. (2006) J. Biol. Chem.     281:11834. The ED    ₅₀ for this effect is 0.5-2.5 µg/mL.

• Source

  Mouse myeloma cell line, NS0-derived

  Human IgG1 (Ser102-Lys330)	IEGR	Human DLEC (Phe46-Ile213) Accession # Q8WTT0
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ser102 (Fc)

• Predicted Molecular Mass

  46 kDa

• SDS-PAGE

  55-61 kDa, reducing conditions

Background: DLEC/CLEC4C/BDCA-2

Dendritic cell lectin (DLEC), also known as BDCA-2, CD303, HECL, and CLEC4C/CLECSF11/CLECSF7, is a 38 kDa type II transmembrane protein in the C-type lectin family (1). Mature human DLEC consists of a 21 amino acid (aa) cytoplasmic domain, a 23 aa transmembrane segment, and a 169 aa extracellular domain (ECD) that contains a juxtamembrane neck region and one carbohydrate recognition domain (CRD) (2, 3). Alternate splicing may generate multiple isoforms that lack the transmembrane segment and/or portions of the cytoplasmic, neck, and CRD regions (2 - 4). An ortholog of human DLEC has not been described in mouse or rat. DLEC expression is restricted to plasmacytoid dendritic cells (pDC) and is downregulated during their maturation (2, 3, 5). pDC play a role in the innate immune response by producing IFN-alpha / beta following exposure to TLR7 and TLR9 agonists such as microbial CpG DNA (3, 5 - 8). Antibody ligation of DLEC on pDC attenuates the CpG-stimulated production of interferons as well as a Th1 biased response (3, 5 - 9). DLEC interactions with HIV-1 gp120 and hepatitis B virus soluble antigen may therefore limit the pDC antiviral response (10, 11). Similar to other C-type lectins, DLEC can mediate antigen uptake for MHC loading and presentation to T cells (3, 12). Crosslinking of DLEC on CpG-stimulated pDC inhibits pDC maturation and induces tyrosine phosphorylation on multiple proteins involved in B cell receptor signaling and endocytosis (5, 7, 8). These functions require the association of DLEC with the ITAM-containing Fc epsilon RI gamma chain (7, 8).

• References:

1. Kanazawa, N. et al. (2004) Immunobiology 209:179.

2. Arce, I. et al. (2001) Eur. J. Immunol. 31:2733.

3. Dzionek, A. et al. (2001) J. Exp. Med. 194:1823.

4. Fernandes, M.J. et al. (2000) Genomics 69:263.

5. Wu, P. et al. (2008) Clin. Immunol. 129:40.

6. Fanning, S.L. et al. (2006) J. Immunol. 177:5829.

7. Rock, J. et al. (2007) Eur. J. Immunol. 37:3564.

8. Cao, W. et al. (2007) PloS Biol. 5:e248.

9. Riboldi, E. et al. (2009) Immunobiology 214:868.

10. Martinelli, E. et al. (2007) Proc. Natl. Acad. Sci. 104:3396.

11. Xu, Y. et al. (2009) Mol. Immunol. 46:2640.

12. Jaehn, P.S. et al. (2008) Eur. J. Immunol. 38:1822.

• Long Name:

  Dendritic Cell Lectin

• Entrez Gene IDs:

  170482 (Human)

• Alternate Names:

  BDCA2; BDCA-2; BDCA2MGC125791; blood dendritic cell antigen 2 protein; Blood dendritic cell antigen 2; CD303 antigen; CD303; CLEC4C; CLECSF11; CLECSF11PRO34150; CLECSF7; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 11; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 7; C-type lectin domain family 4 member C; C-type lectin superfamily member 7; dendritic cell lectin b; Dendritic lectin; DLEC; DLECMGC125792; HECL; HECLMGC125793; MGC125789; PRO34150