• Species Reactivity

  Human

• Specificity

  Detects human IFN‑ beta.

• Source

  Polyclonal Sheep Serum

• Purification

  N/A

• Immunogen

  Recombinant human IFN‑ beta 1a (expressed in mammalian cells)

• Formulation

  Neat serum.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Neutralization

  One neutralization unit is the amount of antiserum required to neutralize one unit ofhuman interferon beta (Hu-IFN-beta ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibitionassay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons,"     J. Virol. 37,755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect InhibitionAssay for Interferon," in     Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In thisantiviral assay for interferon about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%.The units are determined with respect to the international reference standard for Hu-IFN-beta provided by the NationalInstitutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in     Methods in Enzymology(S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for effective neutralizationof Hu-IFN-beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

• Long Name:

  Interferon beta

• Entrez Gene IDs:

  3456 (Human); 15977 (Mouse); 24481 (Rat)

• Alternate Names:

  Fibroblast interferon; IFBIFNB; IFF; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956