• Species Reactivity

  Mouse

• Specificity

  Detects mouse OSM R beta in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CLC, rhCNTF, rhOSM, recombinant mouse (rm) CT-1, rmIL-6, rmIL-11, or rmLIF is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 118125

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse OSM R beta    
  Glu24-Leu738    
  Accession # O70458

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: OSM R beta

Oncostatin M (OSM) is a member of the IL-6 family of cytokines that share the gp130 as a common signal transducing receptor subunit. Human OSM signals through two types of human OSM receptor complexes: the type I complex comprising the leukemia inhibitory factor receptor beta (LIF R beta ) and gp130, the type II complex made up of OSM receptor beta (OSM R beta ) and gp130. In contrast, mouse OSM signals only through the mouse OSM R beta and gp130 complex. Mouse OSM R beta cDNA encodes a 971 amino acid (aa) type I transmembrane protein which contains a 23 aa signal peptide, an extracellular domain of 714 aa, a transmembrane domain of 20 aa and a 214 aa cytoplasmic domain. Mouse OSM R beta alone binds mOSM with low-affinity but forms a high-affinity binding complex in the presence of gp130. Mouse OSM R beta is 55% identical at the amino acid sequence level to human OSM R beta.

• References:

1. Lindberg, R.A. et al. (1998) Mol. Cell. Biol. 18:3357.

2. Tanaka, M. et al. (1999) Blood 93:804.

• Long Name:

  Oncostatin M Receptor beta

• Entrez Gene IDs:

  9180 (Human); 18414 (Mouse); 310132 (Rat)

• Alternate Names:

  OSM R beta; OSMR; OSMRB