Human/Mouse Sonic Hedgehog/Shh N-Terminus PE MAb (171018) 100 TESTS

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Human/Mouse Sonic Hedgehog/Shh N-Terminus PE MAb (171018) 100 TESTS信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human, Mouse

    • Specificity

      Detects human and mouse Sonic Hedgehog/SHH in Western blots and mouse Sonic Hedgehog/SHH in ELISAs. In Western blots, approximately 50% cross-reactivity with recombinant mouse (rm) Desert Hedgehog/Dhh (N‑terminus; aa 23-198) is observed and no cross-reactivity with rmIndian Hedgehog/Ihh (N-terminus; aa 66-240), rmDesert Hedgehog/Dhh (C‑terminus; aa 199-396), or rmSonic Hedgehog/Shh (C‑terminus; aa 199-437) is observed.

    • Source

      Monoclonal Rat IgG    2A Clone # 171018

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      E. coli-derived recombinant mouse Sonic Hedgehog/SHH N-terminus    
      Cys25-Gly198 (Lys122Arg)    
      Accession # Q62226

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Phycoerythrin

    Applications

    • Recommended    
      Concentration

      Sample

    • Intracellular Staining by Flow Cytometry

      10 µL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Intracellular Staining by Flow Cytometry      
         

    Detection of Sonic Hedgehog/Shh in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Rat Anti-Human/Mouse Sonic Hedgehog/Shh N-Terminus PE‑conjugated Monoclonal Antibody (Catalog # IC4641P, filled histogram) or isotype control antibody (Catalog # , open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: Sonic Hedgehog/Shh

    Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened.

    • Entrez Gene IDs:

      6469 (Human); 20423 (Mouse)

    • Alternate Names:

      HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS







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