• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-21 in Western blots. Does not cross-react with recombinant human IL‑21, recombinant mouse (rm) IL‑15, or rmIL‑12.

• Source

  Monoclonal Rat IgG    _2B Clone # 149204

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse IL‑21    
  Pro25-Ser146    
  Accession # Q9ES17

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 488

Applications

• Recommended    
  Concentration

  Sample

• Intracellular Staining by Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-21

Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (Novel Interleukin Receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% aa identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma _c) chain appears to play a role in IL-21 R signaling. The IL-21/IL-21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.

• References:

1. Parrish-Novak, J. et al. (2000) Nature 408:57.

2. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.

3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.

4. Asao, H. et al. (2001) J. Immunol. 167:1.

• Long Name:

  Interleukin 21

• Entrez Gene IDs:

  59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)

• Alternate Names:

  IL21; IL-21; IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform