• Species Reactivity

  Human

• Specificity

  This antibody reacts with human IL-18 on Immunoprecipitation.

• Source

  Monoclonal Rat IgG    _2A Clone # 159-12B

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human IL-18/IL-1F4

• Formulation

  100 μg IgG in 100 μL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunoprecipitation

  5 μg/ 0.5 μg recombinant human IL-18

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  This antibody solution is stable for one year from the date of purchase when stored at -20°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

• References:

1. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)

2. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)

3. Wu, C., J. Immunol. 170, 5571-5577 (2003)

4. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)

5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)

6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)

7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)

8. Okamura, H., et al., Nature 378, 88-91 (1995)

• Long Name:

  Interleukin 18

• Entrez Gene IDs:

  3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

• Alternate Names:

  Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18