• Species Reactivity

  Human

• Specificity

  Stains human ULBP-1 cell transfectants. It does not stain cells transfected with ULBP-2 or ULBP-3.

• Source

  Monoclonal Mouse IgG    _2A Clone # 170818

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  BaF3 mouse pro-B cell line transfected with human ULBP-1

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ULBP-1

ULBP-1 is a member of a family of cell-surface proteins that function as ligands for human NKG2D. ULBP-1 has also been described under the names RaeT1I (retinoic acid early transcript), ALCAN-beta, and NKG2DL1. The name ULBP-1 derives from the original identification of three proteins, ULBP-1, -2, and -3, as ligands for the human cytomegalovirus glycoprotein UL16; they were designated UL16 binding proteins (ULBP). The gene for ULBP-1 resides in a cluster of ten related genes, six of which encode potentially functional glycoproteins. Amino acid sequence identity within this family ranges from 30-95%. These proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. They are anchored to the membrane via a GPI-linkage. ULBP-1 and several other family members are known to bind to human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺ alpha beta T cells. Engagement of NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. ULBP-1 is expressed on some tumor cells and has been implicated in tumor surveillance (1-8).

• References:

1. Cosman, D. et al. (2001) Immunity 14:123.

2. Kubin, M. et al. (2001) Eur. J. Immunol. 31:1428.

3. Sutherland, C. et al. (2002) J. Immunol. 168:671.

4. Steinle, A. et al. (2001) Immunogenetics 53:279.

5. Sutherland, C. et al. (2001) Immunol. Rev. 181:185

6. Pende, D. et al. (2002) Cancer Res. 62:6178.

7. Radosavljevic, M. et al. (2002) Genomics 79:114

8. NKG2D and its Ligands, 2002; .

• Long Name:

  UL16 Binding Protein-1

• Entrez Gene IDs:

  80329 (Human); 77777 (Mouse)

• Alternate Names:

  ALCAN-beta; MULT-1; N2DL1; N2DL-1; NKG2D ligand 1; NKG2DL; NKG2DL1; RAET1I; RAET1Ialcan-beta; Retinoic acid early transcript 1I; UL16 binding protein 1; UL16-binding protein 1; ULBP1; ULBP-1