• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD300a/LMIR1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMIR1, 2, 3, 4, 5, 6, recombinant mouse LMIR3, 4, or 5 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 172224

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CD300a/LMIR1    
  Leu28-Arg183    
  Accession # BAC80268

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Allophycocyanin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD300a/LMIR1

CD300a, also known as LMIR1, CMRF-35H, IRp60, CLM-8, and MAIR-I, is a 60 kDa glycoprotein member of the immunoglobulin superfamily (1). Mouse CD300a consists of a 158 amino acid (aa) extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 112 aa cytoplasmic domain that contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and a non-canonical ITIM (2, 3). Within the ECD, mouse CD300a shares 40% and 66% aa sequence identity with human and rat CD300a, respectively. Alternate splicing generates an additional mouse CD300a isoform with a 4 aa deletion following the Ig-like domain (3). In mouse, CD300a is expressed on peripheral eosinophils, mast cells, neutrophils, dendritic cells, macrophages, and some B cells (2‑4). Antibody cross‑linking of CD300a induces phosphorylation of tyrosine residues in the cytoplasmic domain. This leads to the recruitment of phosphatases SHIP, SHP-1, and SHP-2 and inhibition of NK cell, eosinophil, and mast cell activation (2, 3, 5‑7). Cross‑linking of CD300a to other surface proteins such as SCF R or Fc epsilon RI on mast cells, Fc gamma RIIA on neutrophils, or CCR3 on mast cells and eosinophils inhibits downstream signaling from those receptors (4, 8‑10). CD300a cross‑linking also limits the   in vivo activities of these cells with a subsequent reduction of allergic inflammation symptoms (4, 7, 9).

• References:

1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.

2. Kumagai, H. et al. (2003) Biochem. Biophys. Res. Commun. 307:719.

3. Yotsumoto, K. et al. (2003) J. Exp. Med. 198:223.

4. Munitz, A. et al. (2006) J. Allergy Clin. Immunol. 118:1082.

5. Cantoni, C. et al. (1999) Eur. J. Immunol. 29:3148.

6. Munitz, A. et al. (2006) Blood 107:1996.

7. Bachelet, I. et al. (2005) J. Immunol. 175:7989.

8. Bachelet, I. et al. (2008) J. Immunol. 180:6064.

9. Bachelet, I. et al. (2006) J. Allergy Clin. Immunol. 117:1314.

10. Alvarez, Y. et al. (2008) Mol. Immunol. 45:253.

• Entrez Gene IDs:

  11314 (Human); 217303 (Mouse)

• Alternate Names:

  CD300a antigenCMRF35H9; CD300a molecule; CD300a; CLM8; CLM-8; CMRF35H leukocyte immunoglobulin-like receptor; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF-35-H9CD300 antigen-like family member A; CMRF35HIRp60; CMRF35-like molecule 8; IGSF12; IGSF12NK inhibitory receptor; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4