• Species Reactivity

  Human

• Specificity

  Detects human Resistin in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse RELM alpha.

• Source

  Monoclonal Mouse IgG    _2B Clone # 184320

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Resistin    
  Accession # Q9HD89

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Resistin    
  under non-reducing conditions only

• 
  

• Human Resistin Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human Resistin Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.5-2.0 µg/mL 

  Human Resistin Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human Resistin Protein, CF (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Resistin

Resistin (resistance-to-insulin), also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a 10 kDa member of a small family of secreted cysteine-rich peptide hormones. These molecules purportedly play some role in inflammation, glucose metabolism, and angiogenesis (1-4). Human Resistin precursor is 108 amino acids (aa) in length. It contains an 18 aa signal sequence plus a 90 aa mature region. The mature region shows an N-terminal alpha -helical tail (aa 23-44) and a C-terminal beta -sheet globular head (aa 47-108) (5-7). The Resistin molecule circulates as either a noncovalent trimer (minor form), or a disulfide-linked homohexamer (major form). Noncovalent trimers are generated when the alpha -helical segments self-associate to form a three-stranded coiled-coil structure. Covalent hexamers subsequently appear when the free Cys at position #26 is engaged by adjacent trimers. It is hypothesized that the hexamer is the inactive form of the molecule, and bioactivity is achieved at the target site by disulfide bond reduction (5). Although Resistin family molecules can noncovalently interact to form heterotrimers   in vitro, there is no evidence to suggest this occurs   in vivo with Resistin (8, 9). Mature human Resistin shares 56% and 54% aa identity with mouse and rat Resistin, respectively. Rat Resistin possesses an alternate start site at Met48; this Met is not found in the mouse molecule, however (10). Rodent resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium (6, 11, 12). Although the function of Resistin is unclear, it would seem to block insulin-stimulated uptake of glucose by adipocytes and promote glucose release by hepatocytes (6, 13, 14). As such, it has been proposed to participate in diet-induced insulin-sensitivity. Diets high in fat promote an increase in overall adipocyte size. Hypertrophic adipocytes are known to secrete TNF-alpha which acts locally to block ACRP30 production. Since ACRP30 is an insulin-sensitizer, a drop in ACRP30 availability leads to insulin-  insensitivity, which drives increased insulin production (a compensatory mechanism). High insulin induces Resistin secretion which now antagonizes insulin action, prompting more insulin production and more Resistin secretion (15).

• References:

1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.

2. Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.

3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.

4. King, I.A. et al. (1993) Genomics 18:185.

5. Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.

6. King, I.A. et al. (1996) J. Invest. Dermatol. 107:531.

7. Nuber, U.A. et al. (1996) Eur. J. Cell Biol. 71:1.

8. Chidgey, M. et al. (2001) J. Cell Biol. 155:821.

9. Khan, K. et al. (2006) Br. J. Cancer 95:1367.

10. Hashimoto, T. et al. (1997) J. Invest. Dermatol. 109:127.

11. Caubet, C. et al. (2004) J. Invest. Dermatol. 122:1235.

12. Descargues, P. et al. (2006) J. Invest. Dermatol. 126:1622.

• Entrez Gene IDs:

  56729 (Human); 57264 (Mouse)

• Alternate Names:

  Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1