• Species Reactivity

  Human

• Specificity

  Detects human B7-H3 in direct ELISAs and Western blots. In Western blots, does not cross-react with recombinant human (rh) B7‑H1, rhB7‑H2, rhB7‑1, rhB7‑2, or recombinant mouse PD-L2.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 185504

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human B7‑H3    
  Leu29-Pro245    
  Accession # NP_079516

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B7-H3

B7-H3 is a member of the B7 family of immune costimulatory receptors. Its expression is induced on dendritic cells and monocytes by inflammatory cytokines.

• Long Name:

  B7 Homolog 3

• Entrez Gene IDs:

  80381 (Human); 102657 (Mouse); 102131295 (Cynomolgus Monkey)

• Alternate Names:

  B7H3; B7-H3; B7H34Ig-B7-H3; B7-H3B7 homolog 3; CD276 antigen; CD276 molecule; CD276; Costimulatory molecule